This open-access textbook offers a comprehensive introduction to Space Syntax theory and methodology, tailored for graduate students and researchers. Written in response to the growing need for a dedicated textbook on Space Syntax, it provides a step-by-step guide on applying the Space Syntax method in urban planning and design. The textbook aims to make the Space Syntax approach accessible to a wide range of graduate students and researchers engaged with the built environment, including those in architecture, urban design, urban planning, urban sociology, urban geography, archaeology, road engineering, and environmental psychology. Adopting a didactic approach, each chapter is designed to explain key concepts, illustrate them with practical examples, present the underlying theory, and offer exercises to support learning. The structure of the book gradually introduces fundamental concepts and progressively leads readers to more advanced theories and applications.